Estimate relative activities of transcriptional regulators from transcriptome data by weighted Parametric Gene Set Analysis: wPGSA.
It takes a few minutes or longer depend on your data size.
Log fold change data should be prepared in an ASCII tab delimited text file. It is organized as follows.
To create and edit the log fold change file, use a text editor or Excel. When you use Excel, be aware of a problem as described in Zeeberg et al 2004 .
The first line contains the identifiers for each sample in the dataset.
The remainder of the lines contains log fold change (LFC) data for each of the genes.